What is the OD450 value?

  • Solus assays result in the production of a yellow coloured result
  • The OD value is measure of how much of the yellow colour has been produced.
  • The concentration of colour produced is proportional to the amount of pathogen that was present in the sample.
  • Results are expressed as Optical Density (OD450) measurements using a microplate reader with a 450nm filter.
  • Assay acceptance criteria are detailed in the relevant pack inserts.
  • The value of the blanking well (usually A1) should always be subtracted.